Cara Membuat Reagen Folin Ciocalteu

Cara Membuat Reagen Folin Ciocalteu. PDF fileThe total phenolic extract was determined by the FolinCiocalteu method obtained by total phenolic content of 11968 mgGAE / g for young leaves and 21022 mgGAE / g for the older leaves Antioxidant activity was analyzed by DPPH method obtained IC50.

Ciocalteu Reagen FolinCiocalteu digunakan karna kadar senyawa fenolik Bahasan ini dimulai dengan mengemukakan cara membuat kurva titrasi dan.

Penetapan Kadar Protein dengan Metode Lowry catatan kecil

Reagen FolinCiocalteu 1N Ke dalam labu alas bulat 1500 mL masukkan 100 g sodium tungstate 25 g sodium molibdate 700 mL akuades 50 mL asam phosphate dan 100 mL HCl Campuran direfluks selama 10 jam tambahkan 150 mL lithium sulfat 50 mL akuades dan beberapa tetes bromine (Br2).

BAB I PENDAHULUAN A. Latar Belakang Masalah

reagen E (Folin Ciocalteu) 3 tetes lalu dihomogenkan dengan vortex didiamkan selama 30 menit pada suhu kamar setelah itu diukur absorbansinya pada panjang gelombang 450 – 610 nm.

BAB III METODE PENELITIAN A. Desain Penelitian

OverviewPhysiologic significanceExternal linksThe Folin–Ciocâlteu reagent (FCR) or Folin’s phenol reagent or Folin–Denis reagent also called the gallic acid equivalence method (GAE) is a mixture of phosphomolybdate and phosphotungstate used for the colorimetric in vitro assay of phenolic and polyphenolic antioxidants It is named after Otto Folin Vintilă Ciocâlteu and Willey Glover Denis The FolinDenis reagent is prepared by mixi Text under.